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axopatch 200b amplifier  (Danaher Inc)


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    Structured Review

    Danaher Inc axopatch 200b amplifier
    Axopatch 200b Amplifier, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 21512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/patch+clamp+amplifier+axopatch+200b/Axopatch+200B-2+capacitor+feedback+patch+clamp+amplifier/bio_rxiv__64898__2026__03__05__709926-37-5-15
    Average 97 stars, based on 21512 article reviews
    axopatch 200b amplifier - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Sampling:

    Article Title: Solid-State Nanopore Sizing for cfDNA Sample Quality Control in Point-of-Need Sequencing
    Article Snippet: .. All ionic current-time (I-t) data were acquired at 100 kHz sampling frequency using a patch clamp amplifier Axopatch 200B by Molecular Devices, an NI 6363 DAQ card, and a low pass filter (5 kHz) in a custom LabVIEW program. .. Data was further analyzed using custom MATLAB and Python scripts to extract peak information, and process for multi-gaussian fittings respectively.

    Article Title: Hydrogel interfaced glass nanopore for high-resolution sizing of short DNA fragments.
    Article Snippet: Solid-state nanopores, known for their label-free detection and operational simplicity, face challenges in accurately sizing the short nucleic acids due to fast translocation and a lack of enzyme-based control mechanisms as compared to their biological counterparts with sizing resolutions still limited to ≥100 bp.. Here, we present a facile polyethylene glycol-dimethacrylate (PEG-DMA) hydrogel interfaced glass nanopore (HIGN) system by inserting glass nanopore into the hydrogel to achieve sub-100 base pair (bp) resolution in short DNA sizing analysis.. We systematically investigated the effects of hydrogel mesh size, spatial configurations of glass nanopores about the hydrogel, applied bias voltage, and analyte concentration on the transport dynamics of 200 bp double-stranded DNA (dsDNA).

    Patch Clamp:

    Article Title: Solid-State Nanopore Sizing for cfDNA Sample Quality Control in Point-of-Need Sequencing
    Article Snippet: .. All ionic current-time (I-t) data were acquired at 100 kHz sampling frequency using a patch clamp amplifier Axopatch 200B by Molecular Devices, an NI 6363 DAQ card, and a low pass filter (5 kHz) in a custom LabVIEW program. .. Data was further analyzed using custom MATLAB and Python scripts to extract peak information, and process for multi-gaussian fittings respectively.

    Article Title: Annexin A5 controls VDAC1-dependent mitochondrial Ca 2+ homeostasis and determines cellular susceptibility to apoptosis
    Article Snippet: Mitochondria were approached by Sensapex micromanipulator (SMX series, Sensapex, Oulu, Finland). .. Currents were recorded using a patch-clamp amplifier Axopatch 200B (Molecular Devices, Sunnyvale, CA, USA). .. Data collection was performed using Clampex software of pClamp (V9.0, Molecular Devices, Sunnyvale, CA, USA).

    Article Title: KCa3.1 Inhibition Abrogates Suppression of Cell Migration and F-Actin Assembly Caused by Selective PIEZO1 Activation in Transformed Mouse Fibroblasts
    Article Snippet: Selective SK blocker apamin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was dissolved in distilled water (to 100 μM stock) and was added at 200 nM final concentration to the patch pipette prior to patch-clamp experiments. .. The electrophysiology setup was based on the patch-clamp amplifier Axopatch 200B and analog-to-digital converter Axon Digidata 1550A (Molecular Devices LLC, San Jose, CA, USA), controlled by a personal computer with Axon PClamp 10.7 Software Suite (Molecular Devices LLC, San Jose, CA, USA) for data registration, processing, and analysis. .. Pipettes were pulled from borosilicate glass capillaries with filament (BF-150-86-10, Sutter Instruments, Novato, CA, USA); the resistance was ~5–10 MΩ when filled with a working solution.

    Article Title: Selective identification of allosteric inhibitors and co-drug combinations targeting kinases by using a nanopore tweezer approach
    Article Snippet: .. Ion flow through the nanopore was monitored in voltage-clamp mode by an integrated patch clamp amplifier Axopatch 200B (Molecular Devices). .. The current signal was acquired by an analog-to-digital converter Digidata 1440A (Molecular Devices) at a sampling rate of 10 kHz after processing with a 4-pole lowpass Bessel filter at 2 kHz.

    Article Title: The Sigma-1 receptor agonist PRE084 improves cardiopulmonary function and remodelling in an experimental model of pulmonary arterial hypertension.
    Article Snippet: In RV, total protein bands were reversibly stained with Fast Green FCF and imaged using the Amersham ImageQuant 800 system (GE Healthcare Life Sciences) for quantification. .. Electrophysiological experiments were performed using the wholecell configuration of the patch-clamp technique, with a patch-clamp amplifier Axopatch-200B (Molecular Devices) and were stored on a personal computer with a Digidata 1322 A analogue-to-digital converter (Molecular Devices). .. PCLAMP 10 software (Molecular Devices) was used for both data acquisition and analysis.

    Article Title: Hydrogel interfaced glass nanopore for high-resolution sizing of short DNA fragments.
    Article Snippet: Solid-state nanopores, known for their label-free detection and operational simplicity, face challenges in accurately sizing the short nucleic acids due to fast translocation and a lack of enzyme-based control mechanisms as compared to their biological counterparts with sizing resolutions still limited to ≥100 bp.. Here, we present a facile polyethylene glycol-dimethacrylate (PEG-DMA) hydrogel interfaced glass nanopore (HIGN) system by inserting glass nanopore into the hydrogel to achieve sub-100 base pair (bp) resolution in short DNA sizing analysis.. We systematically investigated the effects of hydrogel mesh size, spatial configurations of glass nanopores about the hydrogel, applied bias voltage, and analyte concentration on the transport dynamics of 200 bp double-stranded DNA (dsDNA).

    Article Title: KCa3.1 Inhibition Abrogates Suppression of Cell Migration and F-Actin Assembly Caused by Selective PIEZO1 Activation in Transformed Mouse Fibroblasts.
    Article Snippet: Selective SK blocker apamin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was dissolved in distilled water (to 100 μM stock) and was added at 200 nM final concentration to the patch pipette prior to patch-clamp experiments. .. The electrophysiology setup was based on the patch-clamp amplifier Axopatch 200B and analog-to-digital converter Axon Digidata 1550A (Molecular Devices LLC, San Jose, CA, USA), controlled by a personal computer with Axon PClamp 10.7 Software Suite (Molecular Devices LLC, San Jose, CA, USA) for data registration, processing, and analysis. .. Pipettes were pulled from borosilicate glass capillaries with filament (BF-150-86-10, Sutter Instruments, Novato, CA, USA); the resistance was ~5–10 MΩ when filled with a working solution.

    Article Title: Annexin A5 controls VDAC1-dependent mitochondrial Ca 2+ homeostasis and determines cellular susceptibility to apoptosis.
    Article Snippet: Mitochondria were approached by Sensapex micromanipulator (SMX series, Sensapex, Oulu, Finland). .. Currents were recorded using a patch-clamp amplifier Axopatch 200B (Molecular Devices, Sunnyvale, CA, USA). .. Data collection was performed using Clampex software of pClamp (V9.0, Molecular Devices, Sunnyvale, CA, USA).

    Software:

    Article Title: KCa3.1 Inhibition Abrogates Suppression of Cell Migration and F-Actin Assembly Caused by Selective PIEZO1 Activation in Transformed Mouse Fibroblasts
    Article Snippet: Selective SK blocker apamin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was dissolved in distilled water (to 100 μM stock) and was added at 200 nM final concentration to the patch pipette prior to patch-clamp experiments. .. The electrophysiology setup was based on the patch-clamp amplifier Axopatch 200B and analog-to-digital converter Axon Digidata 1550A (Molecular Devices LLC, San Jose, CA, USA), controlled by a personal computer with Axon PClamp 10.7 Software Suite (Molecular Devices LLC, San Jose, CA, USA) for data registration, processing, and analysis. .. Pipettes were pulled from borosilicate glass capillaries with filament (BF-150-86-10, Sutter Instruments, Novato, CA, USA); the resistance was ~5–10 MΩ when filled with a working solution.

    Article Title: KCa3.1 Inhibition Abrogates Suppression of Cell Migration and F-Actin Assembly Caused by Selective PIEZO1 Activation in Transformed Mouse Fibroblasts.
    Article Snippet: Selective SK blocker apamin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was dissolved in distilled water (to 100 μM stock) and was added at 200 nM final concentration to the patch pipette prior to patch-clamp experiments. .. The electrophysiology setup was based on the patch-clamp amplifier Axopatch 200B and analog-to-digital converter Axon Digidata 1550A (Molecular Devices LLC, San Jose, CA, USA), controlled by a personal computer with Axon PClamp 10.7 Software Suite (Molecular Devices LLC, San Jose, CA, USA) for data registration, processing, and analysis. .. Pipettes were pulled from borosilicate glass capillaries with filament (BF-150-86-10, Sutter Instruments, Novato, CA, USA); the resistance was ~5–10 MΩ when filled with a working solution.



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